The Redprobes assay for heparin and heparan sulphate (HS) is based on a novel fluorescent dye, Heparin Red, developed by Professor Roland Kramer at The University of Heidelberg in Germany.
In complex with heparin or HS the red dye fluorescence is reduced and this property underpins a sensitive (about 1µg/ml detection) and rapid analytical technique suitable for many applications in biomedical research and industrial processes.
The assay comes in two microtitre-based formulations, the Heparin Red Kit for measuring heparin or HS in plasma and the Heparin Red Ultra with potential in various applications such as monitoring the commercial purification of heparin and for the analysis of heparin in urine and other biological fluids; the assays are suitable for low molecular weight heparins, including enoxaparin, heparin oligosaccharides and are independent of the anticoagulant activity of heparin.
Application and Information
Heparin Red Kit
Designed and optimised for measuring heparins in human plasma
Rapid mix-and-read fluorescence assay in microtitre plates
Sensitive detection in 20ul of plasma (low ug/ml range)
Independent of anticoagulant activity of heparins
Suitable for pharmacokinetic studies of emerging, new heparin-based therapies
Stable, protein-free reagents
Warttinger U. et al., A fluorescent probe assay (Heparin Red) for direct detection of heparins in human plasma Anal. Bioanal. Chem. 2016, 408, 8241-8251.
Warttinger, U &, Krämer, R. Instant determination of the potential biomarker heparan sulfate in human plasma by a mix-and-read fluorescence assay.
Galli M. et al., Roneparstat (SST0001), an innovative heparanase (HPSE) inhibitor for multiple myeloma (MM) therapy: first in man study. Blood 2015, 126, 3246-3246.
Heparin Red Ultra Kit
Optimised for rapid detection of heparins and heparan sulphate (HS) in complex solutions Simple mix-and-read fluorescence assay in microtitre plates Small sample volumes, analysis of low heparin (ug/ml) concentrations Suitable for heparin analysis in urine Stable protein-free reagents
Reference* Wattinger U, Kramer R. Quantification of heparin in complex matrices (including urine) using a mix-and-read fluorescence assay.
* Iduron and Redprobes are not in any way responsible for the content and accuracy of the papers listed above.