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Heparan Sulphate Oligosaccharides

Description

Heparan Sulphate (HS) is a near-universal co-receptor for many growth factors and morphogenic proteins; it has a complex domain organisation of alternating high and low sulphated regions (Int J Exp Pathol (2015). 96:203-231.) in which variations in polymer sulphation and epimerisation lead to considerable structural diversity designed for selective binding to its many client proteins. We take advantage of the substrate specificities of Heparinase I (Hep I) and Heparinase III (Hep III) for the preparation of HS-oligos from regions of low and high sulphation in HS.

Separation of HS-oligosaccharides (HS-oligos) in the enzyme digests is achieved by high resolution preparative gel filtration of the Hep I or Hep III enzyme digests. The separated HS-oligo fractions are then analysed for disaccharide composition and molecular size homogeneity. Although substantially homogeneous by SEC Superdex S-75, HS-oligos in each size class contain sequences that vary in pattern and density of sulphation and there may be some limited overlap with oligos in adjacent size groups.

HS-oligos. from both Hep I and Hep III digests are available in the size range from dp4 to dp20 where dp is degree of polymerisation, a dp4 is a tetrasaccharide, dp6 a hexasaccharide, dp8 an octasaccharide etc. up to dp20.

High mol wt fractions (>6000 daltons) are also available.


Heparinase IHS-Oligos:Hep I

Enzyme specificity is for the NS-amino sugar linkage to 2-O-sulphated iduronic acid;

GlcNS (+/-6S) – IdoA,2S

Hep I acts exclusively in the sulphated regions of HS, the S-domains, resulting in oligosaccharides of low/intermediate sulphation that resist the action of this enzyme. These regions comprise over 50% of the HS chain, and include the N- and 6-O sulphated transition zones, but there is a dearth of information on their conformational features and biological properties. A simplified, general formula for HS-Oligos produced by Hep I is:

*UA,2S – **GlcNS, – [GlcA – GlcNAc]n=0-to-8 – IdoA  – **GlcNS

*UA (uronic acid) at the non-reducing end contains a C4/C5 double bond due the eliminative action of Hep I

**amino sugars may be sulphated at C6

Heparinase IIIHS-Oligos:Hep III

Enzyme specificity is for the amino sugar linkage to glucuronic acid:

GlcNAc/GlcNS – GlcA

Hep III targets the low sulphated regions of HS; oligos excised by Hep III are enriched in sulphated sequences derived from the more highly sulphated S-domains of HS.  A simplified, general formula for HS-oligos produced by Hep III is:

*UA – GlcNS - [IdoA (+/-2S) – **GlcNS]n=0-to-8 – IdoA  – GlcNAc

*UA (uronic acid) at the non-reducing end contains a C4/C5 double bond due the eliminative action of Hep III

**amino sugars may be sulphated at C6

 Merry et al., (1999), J. Biol. Chem. 274, 18455-18462

Product Catalog No Qty £
  1. Heparan Sulphate Oligosaccharides. Heparinase I Digestion. Low/Intermediate Sulphation.
    Heparinase I Heparan Sulphate Oligosaccharides dp4 Hep I HSO04 1mg 150.00 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp6 Hep I HSO06 1mg 150 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp8 Hep I HSO08 1mg 150.00 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp10 Hep I HSO10 1mg 150 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp12 Hep I HSO12 1mg 150 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp14 Hep I HSO14 1mg 150 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp16 Hep I HSO16 1mg 150 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp18 Hep I HSO18 1mg 150 Enquire Now
    Heparinase I Heparan Sulphate Oligosaccharides dp20 Hep I HSO20 1mg 150 Enquire Now
  2. Heparan Sulphate Oligosaccharides. Heparinase III Digestion. High Sulphation.
    Heparinase III Heparan Sulphate Oligosaccharides dp4 Hep III HSO04 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp6 Hep III HSO06 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp8 Hep III HSO08 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp10 Hep III HSO10 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp12 Hep III HSO12 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp14 Hep III HSO14 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp16 Hep III HSO16 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp18 Hep III HSO18 1mg 150 Enquire Now
    Heparinase III Heparan Sulphate Oligosaccharides dp20 Hep III HSO20 1mg 150 Enquire Now

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