Our Products

Heparins

We offer a unique range of heparins, heparin saccharides and defined sequences

Heparin - Guidance notes

Heparin is a highly-sulphated glycosaminoglycan found mainly in mast cell granules; it’s principal clinical use is as an anticoagulant but its reactivity with many effector proteins is leading to potential applications in a variety of human diseases. Heparin is chemically-related to heparan sulphate (HS), a common component of cell surfaces that plays key roles in the control of cell growth and development. Heparin, and oligosaccharides derived from heparin, can be used as functional analogues of the sulphated regions of HS but with the proviso that the high charge density of heparin may, in some instances, lack the discrimination of protein binding seen in the less sulphated HS. To some degree this limitation can be overcome by use of size-limited heparin saccharides and by the preparation of specifically-desulphated heparin derivatives, all available from Iduron and listed below; such key reagents can give valuable information on the structural elements necessary for the activity under study. If more in depth work is needed then data acquired using our heparin-based reagents can guide the selection of heparin and HS motifs in our extensive “HepMer” range of defined oligomeric sequences.

Page, C. (2013 Heparin and Related Drugs: beyond Antocoagulant Activity. ISRN Pharmacology, 2013; ID 910973

Cassinelli, G. and Naggi, A. (2016) Old and new applications of Non-Antocoagulant Heparin. Int. Cardiol. 21251: S14-S21

Kjellen, L. and Lindahl, U. (2018) Specificity of glycosaminoglycan-protein interactions. Current Opinion in Structural Biology 50:101–108

Gallagher J. (2015) Fell-Muir Lecture: Heparan Sulphate and the art of cell regulation: a polymer chain conducts the protein orchestra. Int J Exp Pathol. 96:203-231.

Product info:

Heparin and Low Molecular Weight Heparin

Heparin Polymer Molecular Weight (Mw) Series prepared from High Grade Unfractionated Heparin (UF)

These highly-purified, low polydispersity heparins were isolated from our High-Grade Heparin (Cat. No. HEP-HG 001; Mw 15,700) and cover the range of polymer chains present in unfractionated heparins.

Molecular weights were determined using the USP Heparin Sodium molecular weight calibrant for molecular weight measurement of unfractionated heparin. 


Selectively
 Desulphated Heparin Polymers

These heparin products have been made from high quality heparin modified by standard chemical methods to selectively remove sulfate groups from C2 of Iduronate, (De2S Hep), C6 of glucosamine (De6SHep) or the N-sulfate of Glucosamine (DeNS Hep). The DeNS heparin contains the free amino group (NH+3); in DeNS/Ac Hep the free amino group has been modified by acetylation.


Heparin Oligosaccharides

Prepared by controlled partial depolymerisation of UF heparin by Heparinase I and separation of the derived oligosaccharides using high resolution gel filtration. The products cover the entire range of oligosaccharides present in Low Molecular Weight Heparins. General formula:

*UA,2S – (GlcNS,6S – IdoA,2S) – GlcNS,6S

*the uronic acid at the non-reducing end of the chain contains a C4-C5 double bond due to the endolytic action of Heparinase I

Although the main disaccharide in heparin is the trisulphated unit GlcNS,6S – IdoA,2S heparin also contains di-, mono- and non-sulphated units; thus each size class of oligosaccharide, though substantially homogeneous in molecular size, contains structures that vary in content and pattern of sulphation. The 2mg amounts of supplied material can be subfractionated (eg. by SAX-hplc) for further analysis of naturally-occurring heparin sequences within a specific size class or classes.

Main Heparin Disaccharide Unit

heparin-oligosaccharide-disacc-unit.gif#asset:1663


Selectively Desulphated Heparin Oligosaccharides

Heparin oligosaccharides made by partial digestion of high quality heparin modified by standard chemical methods to selectively remove the sulphate groups.

Investigate the specific protein binding motifs in Heparin and HS GAGs. Use these products in conjunction with our heparin oligosaccharides and HepMers to investigate the essential functional groups and specific oligosaccharide chain length required for high affinity/optimal protein interactions.

4 variations of selectively desulphated heparin oligosaccharides are available, dp4 – dp12:

2-O-desulphated heparin oligosaccharides Sulphate group removed from the C2 of iduronate

6-O-desulphated heparin oligosaccharides Sulphate group removed from the C6 of glucosamine

N-desulphated heparin oligosaccharides Sulphate group removed from the N sulphate of the glucoasmine

N-desulphated re N-acetylated heparin oligosaccharides The free amino group (NH+3) in N-desulphated heparin has been modified by acetylation


Product Catalog No Qty £
  1. Heparin Polymers from High Grade UF Heparin
    High Grade Heparin Mw 15,700 HEP-HG 100 2mg 90.00 Enquire Now
    Heparin Polymer Mw 10,980 HEP-POLY 1 1mg 140.00 Enquire Now
    Heparin Polymer 2 Mw 13030 HEP-POLY 2 1mg 140.00 Enquire Now
    Heparin Polymer 3 Mw 14,580 HEP-POLY 3 1mg 85.00 Enquire Now
    Heparin Polymer 4 Mw 16,400 HEP-POLY 4 1mg 85.00 Enquire Now
    Heparin Polymer 5 Mw 18,730 HEP-POLY 5 1mg 85.00 Enquire Now
    Heparin Polymer 6 Mw 22,210 HEP-POLY 6 1mg 140.00 Enquire Now
  2. Selectively Desulphated Heparins
    2-O-Desulphated Heparin DSH001/2 2mg 125.00 Enquire Now
    6-O-Desulphated Heparin DSH002/6 2mg 150.00 Enquire Now
    N Desuphated Heparin DSH003/N 2mg 100.00 Enquire Now
    N-Desulphated re N-Acetylated Heparin DSH004/Nac 2mg 150.00 Enquire Now
  3. Heparin Oligosaccharides
    Heparin Oligosaccharide dp4 HO04 2mg 205.00 Enquire Now
    Heparin Oligosacharide dp6 HO06 2mg 205.00 Enquire Now
    Heparin Oligosaccharide dp8 HO08 2mg 205.00 Enquire Now
    Heparin Oligosaccharide dp10 HO10 2mg 205.00 Enquire Now
    Heparin Oligosaccharide dp12 HO12 2mg 205.00 Enquire Now
    Heparin Oligosaccharide dp14 HO14 2mg 205.00 Enquire Now
    Heparin Oligosaccharide dp16 HO16 2mg 205.00 Enquire Now
    Heparin Oligosaccharide dp18 HO18 2mg 205.00 Enquire Now
    Heparin Oligosaccharide dp20 HO20 2mg 205.00 Enquire Now
    Heparin Saccharide Av. Mol. Wt. 6300 HO22 2mg 205.00 Enquire Now
    Heparin Saccharide Av. Mol. Wt. 6850 HO24 2mg 205.00 Enquire Now
    Heparin Saccharide <9000 HO30 2mg 205.00 Enquire Now
  4. Desulphated Heparin Oligosaccharides
    2-O-Desulphated Heparin Oligo dp4 2-O DSHO04 1mg 125.00 Enquire Now
    2-O-Desulphated Heparin Oligo dp6 2-O DSHO06 1mg 125.00 Enquire Now
    2-O-Desulphated Heparin Oligo dp8 2-O DSHO08 1mg 125.00 Enquire Now
    2-O-Desulphated Heparin Oligo dp10 2-O DSHO10 1mg 125.00 Enquire Now
    2-O-Desulphated Heparin Oligo dp12 2-O DSHO12 1mg 125.00 Enquire Now
    6-O-Desulphated Heparin Oligo dp4 6-O DSHO04 1mg 150.00 Enquire Now
    6-O-Desulphated Heparin Oligo dp6 6-O DSHO06 1mg 150.00 Enquire Now
    6-O-Desulphated Heparin Oligo dp8 6-O DSHO08 1mg 150.00 Enquire Now
    6-O-Desulphated Heparin Oligo dp10 6-O DSHO10 1mg 150.00 Enquire Now
    6-O-Desulphated Heparin Oligo dp12 6-O DSHO12 1mg 150.00 Enquire Now
    N Desulphated Heparin Oligo dp4 N DSHO04 1mg 100.00 Enquire Now
    N Desulphated Heparin Oligo dp6 N DSHO06 1mg 100.00 Enquire Now
    N Desulphated Heparin Oligo dp8 N DSHO08 1mg 100.00 Enquire Now
    N Desulphated Heparin Oligo dp10 N DSHO10 1mg 100.00 Enquire Now
    N Desulphated Heparin Oligo dp12 N DSHO12 1mg 100.00 Enquire Now
    N-Desulphated re N-Acetylated Heparin Oligo dp4 reAc DSHO04 1mg 150.00 Enquire Now
    N-Desulphated re N-Acetylated Heparin Oligo dp6 reAc DSHO06 1mg 150.00 Enquire Now
    N-Desulphated re N-Acetylated Heparin Oligo dp8 reAc DSHO08 1mg 150.00 Enquire Now
    N-Desulphated re N-Acetylated Heparin Oligo dp10 reAc DSHO10 1mg
    150.00 Enquire Now

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