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Heparin Test kits

Description

Heparin test kits designed for USP and EP compliant testing of Anti-IIa and Anti-Xa activity of heparin and LMW heparin including Enoxaparin Sodium. Supplied by Provision Kinetics, USA.

The versatile, highly reliable kits have a wide range of uses including pharmaceutical applications like quality control or medical device and research testing, as well as test adaptations for heparin-coated devices.

Application

The Kinetichrome heparin test kits are designed for the chromogenic measurement of the anti-factor IIa (thrombin) and/or anti-factor Xa activity in samples containing heparin, low molecular weight heparin and related molecules with thrombin and/or factor Xa inhibitor activity.

A distinctive feature of the Kinetichome product line is that these kits are designed and optimized for activity measurements with buffer as the reaction medium, rather than plasma. Thus, the Kinetichrome heparin test kits are ideally suited for research, industrial, and pharmacopeial test applications where buffer is the specified test medium.

Information

New heparin assay guidelines from European Pharmacopeia.

The 8th edition of the European Pharmacopoeia, supplement 8.3, issued on 1st July 2014 and coming into effect from 1st January 2015 has new guidelines on chromogenic assays for anti-IIa and anti-Xa activities

Provision Kinetics has modified their Xa Heparin test kits in order to FULLY conform with the EP directives – accordingly, the XAE-100 and XAE-200 Heparin Test Kits are available now. The IIa Heparin Test Kits are already compatible the ruling.

Please note

The kits have 100 and 200 determinations when in optimal conditions on an automated haemostasis instrument, if you are using the manual test method(s) on a lab bench it is more realistic to expect about 50 determinations from a 100 determination kit and 100 from a 200 determination kit.

We have programs/applications available for major haemostasis instrument platforms including:
Instrumentation Laboratory (IL) product line
Diagnostica Stago (Stago) line
Sysmex line (Siemens in the US)

Please email info@iduron.co.uk for any questions, a quote or to place an order

Summary and principle

Thrombin (Factor IIa) is the terminal enzyme in the classical coagulation cascade, where it plays roles in both the fibrin deposition and platelet activation processes of blood coagulation. Unfractionated Heparin (UFH) and Low Molecular Weight Heparin (LMW-H) inhibit blood coagulation via initial binding of these molecules to Antithrombin (AT), followed subsequently by the AT-heparin complex inhibiting Factor Ila. In buffer, this same “Anti-lla” activity of heparin can be foJlowed enzymatically by means of a chromogenic peptide substrate which, upon hydrolysis by residual factor IIa, provides a chromophore (405 nm) whose measure is inversely proportional to the initial amount of heparin present in the test sample. The paranitroanaline (pNA) chromophore generated by the residual factor lIa can be measured either kinetically or by an acid-stopped end-point method.

Schematically, the procedure is carried out as follows:

AT + heparin → (AT-heparin)

[AT-heparin) + IIa (known excess) → Ila (residual) + IIa-AT-heparin (inactive)

IIa (residual) + IIa Substrate (colorless) → pNA chromophore (405 nm) + peptide

Summary and Principle

Factor Xa serves a key role in the classical coagulation cascade where it serves as part of the Xase complex that converts pro-thrombin to thrombin. Unfractionated Heparin (UFH) and Low Molecular Weight Heparin (LMW-H) inhibit blood coagulation via initial binding of these molecules to Antithrombin (AT), followed subsequently by the AT-heparin complex inhibiting Factor Xa, as well as thrombin (Factor IIa). In buffer, this same “Anti-Xa” activity of heparin can be followed enzymatically by means of a chromogenic peptide substrate which generates a chromophore (405 nm), whose measure is inversely proportional to the initial amount of heparin present in the test sample. The paranitroanaline (pNA) chromophore generated by the residual factor Xa can be measured either kinetically or by an acid-stopped end-point method.

Schematically, the procedure is carried out as follows:

AT + heparin → [AT-heparin]

[AT-heparin] + Xa (known excess) → Xa (residual) + Xa-AT-heparin (inactive)

Xa (residual) + Xa Substrate (colorless) → pNA chromophore (405 nm) + peptide

Product Catalog No Qty £
  1. Anti XAE kits. USP & EP Compliant
    Anti XAE Assay Kit, 100 determinations XAE 100 1 Kit 205.00 Enquire Now
    Anti XAE Assay Kit, 200 determinations XAE 200 1 Kit 345.00 Enquire Now
  2. Anti IIa kits. USP & EP Compliant
    Anti IIa Assay Kit, 100 determinations IIa 100 1 Kit 190.00 Enquire Now
    Anti IIa Assay Kit, 200 determinations IIa 200 1 Kit 310.00 Enquire Now
  3. Anti Xa kits. , USP Compliant
    Anti Xa Assay Kit, 100 determinations XA 100 1 Kit 205.00 Enquire Now
    Anti Xa Assay Kit, 200 determinations XA 200 1 Kit 345.00 Enquire Now
  4. Heparin sodium reference standard
    Heparin Sodium BRP Ph. Eur. Reference Standard HEP-BRP 1010 IU/mL
    120.00 Enquire Now
  5. Tris-Saline Buffer Concentrate
    Tris-Saline Buffer Concentrate BUF 5X-100 100ml 50.00 Enquire Now

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